奈良県立医科大学

生体分子不均衡制御学

共同研究講座

Laboratory of Bio-Molecular Dynamics, Department of Collaborative Research, Nara Medical University

MEMBER

教授 永森收志  

助教 Wiriyasermkul, Pattama

助教 Kongpracha, Pornparn

特別研究員 森山理美

博士研究員 田中容子

教室職員 坂本千鶴

Professor Shushi Nagamori  

Assistant Professor Pattama Wiriyasermkul

Assistant Professor Pornparn Kongpracha

Special Researcher Satomi Moriyama

Postdoctral Researcher Yoko Tanaka

Secretary Chizuru Sakamoto

 
RESEARCH

研究内容
生命の根源に関わる物質の不均衡分布は、生体膜に存在する物質輸送を司る膜タンパク質である膜輸送体により作られます。私たちは膜輸送体、特にトランスポーターを対象として研究を行っています。また、膜輸送体が他のタンパク質分子と複合体を形成し、機能的に共役していることから、トランスポーターとそれを含む膜タンパク質複合体を研究対象としています。

研究手法
古典的な膜タンパク質の生化学的手法に加え、最新式の定量型質量分析計を用いて研究を進めています。

Life uses concentration gradients of substances across membranes. Disequilibrium of substances is generated and maintained by membrane transport proteins. Our laboratory focuses on the research of membrane proteins, especially transporters. In addition to biochemical approaches, we apply the-state-of-art proteomics to study transport mechanisms and physiological roles of transporters. Along with the studies of transporters, we are also interested in complexes of transporters and their interacting proteins in terms of functional relationship.

Biochemistry by using proteoliposomes or Kabacksomes
Proteomics by using Thermo Q-Exactive



 
WORK

Publications
Selected papers

  1. Ikeda K, Kinoshita M, Kayama H, Nagamori S, Kongpracha P, Umemoto E, Okumura R, Kurakawa T, Murakami M, Mikami N, Shintani Y, Ueno S, Andou A, Ito M, Tsumura H, Yasutomo K, Ozono K, Takashima S, Sakaguchi S, Kanai Y, Takeda K. Slc3a2 mediates branched-chain amino acid-dependent maintenance of regulatory T cells. Cell Rep. 2017;21(7):1824-1838.
  2. Kongpracha P, Nagamori S, Wiriyasermkul P, Tanaka Y, Kaneda K, Okuda S, Ohgaki R, Kanai Y. Structure-activity relationship of a novel series of inhibitors for cancer type transporter L-type amino acid transporter 1 (LAT1). J Pharmacol Sci. 2017;133(2):96-102.
  3. Watabe T, Ikeda H, Nagamori S, Wiriyasermkul P, Tanaka Y, Naka S, Kanai Y, Hagiwara K, Aoki M, Shimosegawa E, Kanai Y, Hatazawa J. 18F-FBPA as a tumor-specific probe of L-type amino acid transporter 1 (LAT1): a comparison study with 18F-FDG and 11C-Methionine PET. Eur J Nucl Med Mol Imag. 2017;44(2):321-331.
  4. Nagamori S, Wiriyasermkul P, Okuda S, Kojima N, Hari Y, Kiyonaka S, Mori Y, Tominaga H, Ohgaki R, Kanai Y. Structure-activity relations of leucine derivatives reveal critical moieties for cellular uptake and activation of mTORC1-mediated signaling. Amino Acids 2016;48:1045-58.
  5. Nagamori S, Wiriyasermkul P, Espino Guarch M, Okuyama H, Nakagomi S, Tadagaki K, Nishinaka Y, Bodoy S, Takafuji K, Okuda S, Kurokawa J, Ohgaki R, Nunes V, Palacín M, Kanai Y. Novel cystine transporter in renal proximal tubule identified as a missing partner of cystinuria-related plasma membrane protein rBAT/SLC3A1. Proc. Natl. Acad. Sci. USA 2016 ; 113(3):775-80.
  6. Ohgaki R, Wei L, Yamada K, Hara T, Kuriyama C, Okuda S, Ueta K, Shiotani M, Nagamori S, Kanai Y. Interaction of the sodium/glucose cotransporter (SGLT) 2 inhibitor canagliflozin with SGLT1 and SGLT2: inhibition kinetics, sidedness of action, and transporter-associated incorporation accounting for its pharmacodynamic and pharmacokinetic features. J Pharmacol Exp Ther. 2016; 358(1):94-102.
  7. Wongthai P, Hagiwara K, Miyoshi Y, Wiriyasermkul P, Wei L, Ohgaki R, Kato I, Hamase K, Nagamori S, Kanai Y. Boronophenylalanine, a boron delivery agent for boron neutron capture therapy, is transported by ATB0,+, LAT1 and LAT2. Cancer Sci. 2015;106(3):279-86.
  8. Uetsuka S#, Ogata G#, Nagamori S#,*, Isozumi N, Nin F, Yoshida T, Komune S, Kitahara T, Kikkawa Y, Inohara H, Kanai Y, Hibino H* #Equal contribution, *Corresponding authors. Molecular architecture of the stria vascularis membrane transport system, which is essential for physiological functions of the mammalian cochlea. Eur J Neurosci.201542(3):1984-2002.
  9. Watashi K, Sluder A, Daito T, Matsunaga S, Ryo A, Nagamori S, Iwamoto M, Nakajima S, Tsukuda S, Borroto-Esoda K, Sugiyama M, Tanaka Y, Kanai Y, Kusuhara H, Mizokami M, Wakita T. Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting a membrane transporter NTCP. Hepatology 2014; 59(5):1726-37.
  10. Moser M, Nagamori S, Huber M, Tokuda H, Nishiyama K. Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation. Proc. Natl. Acad. Sci. USA 2013;110:9734-9.
  11. Khunweeraphong N, Nagamori S, Wiriyasermkul P, Nishinaka Y, Wongthai P, Ohgaki R, Tanaka H, Tominaga H, Sakurai H, Kanai Y. Equal contribution Establishment of stable cell lines with high expression of heterodimers of human 4F2hc and human amino acid transporter LAT1 or LAT2 and delineation of their differential interaction with α-alkyl moieties. J Pharmacol Sci.2012; 119: 368-80
  12. Wiriyasermkul P, Nagamori S, Tominaga H, Oriuchi N, Kaira K, Nakao H, Kitashoji T, Ohgaki R. Tanaka H, Endou H, Endo K, Sakurai H and Kanai Y.Transport of 3-fluoro-L-α-methyl tyrosine by tumor-upregulated amino acid transporter LAT1: a cause of the tumor uptake in PET. J Nucl Med.2012; 53: 1253-61
  13. Matsuo H, Chiba T, Nagamori S, Nakayama A, Domoto H, Phetdee K, Wiriyasermkul P, Kikuchi Y, Oda T, Nishiyama J, Nakamura T, Morimoto Y, Kamakura K, Sakurai Y, Nonoyama S, Kanai Y, Shinomiya N. Mutations in glucose transporter 9 gene SLC2A9 cause renal hypouricemia. Am J Hum Genet. 2008; 83(6): 744-751.
  14. Shimohata N#, Nagamori S#, Akiyama Y, Kaback HR, Ito K. #Equal contribution SecY alterations that impair membrane protein folding and generate a membrane stress.   J Cell Biol. 2007; 176: 307-17.
  15. Bloois EV, Nagamori S, Koningstein G, Ullers RS, Preuss M, Oudega B, Harms N, Kaback HR, Herrmann JM, Luirink J. The Sec-independent function of E. coli YidC is evolutionary conserved and essential. J Biol. Chem.2005; 280(13):12996-3003.
  16. Guan L, Smirnova IN, Verner G, Nagamori S, Kaback HR. Manipulating phospholipids for crystallization of membrane transport proteins. Proc. Natl. Acad. Sci. USA. 2006; 103:1723-6
  17. Nagamori S, Smirnova IN, Kaback HR.  Role of YidC in folding of polytopic membrane proteins J Cell Biol. 2004; 165:53-62
  18. Nagamori S, Vázquez-Ibar JL., Weinglass AB, Kaback HR.  In vitro synthesis of lactose permease to probe the mechanism of membrane insertion and folding.  J Biol Chem. 2003; 278:14820-14826
  19. Nagamori S, Nishiyama K, Tokuda H.  Two SecG molecules present in a single protein translocation machinery are functional even after crosslinking.  J Biochem.2000; 128:129-137
  20. Chida K, Nagamori S, Kuroki T. Nuclear translocation of Fos is stimulated by interaction with Jun through the leucine zipper. Cell Mol Life Sci.1999; 55(2): 297-302.

 




CONTACT

Address
〒634ー8521
奈良県橿原市四条町840番地 奈良県立医科大学
基礎医学棟5階 生体分子不均衡制御学共同研究講座

e-mail
nagamorilab[at]naramed-u.ac.jp

Phone
0744ー22-3051 ex. 2504

 

奈良県立医科大学 生体分子不均衡制御学共同研究講座